Biotic and Abiotic stress
Rahil Dowlatabadi; Hajar Shayesteh; Amin Mirshamsi Kakhki; Mohammad Zare Mehrjerdi; Alireza Seifi
Abstract
Broomrape (Orobanche aegyptica) is a notorious parasitic plant that cause significant production loss. Here we report analysis of publicly available RNA-seq data for broomrape, coupled with experimental verification of part of the results. After quality control of raw illumine reads, qualified reads ...
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Broomrape (Orobanche aegyptica) is a notorious parasitic plant that cause significant production loss. Here we report analysis of publicly available RNA-seq data for broomrape, coupled with experimental verification of part of the results. After quality control of raw illumine reads, qualified reads were mapped against Orobanch transcriptome. Differential gene expression analysis, performed by using DESeq package, identified 391 differentially expressed genes between seed imbibition and haustorium attachment stages. The expression of orthologs of these genes in close relatives of Orobanch, which are parasitic, hemi- or non-parasitic, was investigated. From 391 identified genes, 87 genes showed high levels of expression in parasitic relatives and not in non-parasitic ones. Based on these analyses the 87 genes were considered as candidate genes involved in establishment of parasitic interaction between Orobanch and its host. The expression of nine of these genes were checked experimentally in flower tissues of Orobanch and in tissues sampled from the attachment site on the host root. The expression of Or2094, which a putative serine-carboxy peptidase, was detected only in the attachment site, supporting the role of this gene in establishment of the parasitic interaction. The results of this work will pave the way for future genetic engineering projects to use host-induced gene silencing strategy to enhance resistance to Orobanch in host crop plants.
Biotic and Abiotic stress
V Ghasemi omraan; A Bagheri; GH Nematzadeh; A Mirshamsi; Nadali Babaeian Jelodar
Volume 2, Issue 2 , September 2012, , Pages 27-37
Abstract
Salinity, predominantly NaCl, limits plant growth and impairs agricultural productivity. In higher plants, Na+ efflux and compartmentalization are achieved by Na+/H+ antiporters located in both the plasma and vacuolar membranes. Here we investigated the expression pattern of the genes AlNHX ...
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Salinity, predominantly NaCl, limits plant growth and impairs agricultural productivity. In higher plants, Na+ efflux and compartmentalization are achieved by Na+/H+ antiporters located in both the plasma and vacuolar membranes. Here we investigated the expression pattern of the genes AlNHX and AlSOS1 under 250 mM NaCl treatment after 6h and 1, 3, 8 and 17 days time intervals by Real Time-PCR technique. The transcript levels of AlNHX and al AlSOS1 were up-regulated by salt stress in all tissues. The AlSOS1 expression remarkably increased in leaves after 6 h and the AlNHX transcript abundance reached to the maximum level after 24 h. In node and internode tissues the transcript levels of AlNHX and AlSOS1 increased sharply 24 h after salt treatment and then gradually decreased within 3 and 8 days and finally after 17 days reached to a steady-state in which the mRNA content was similar to that of control plants. The transcript abundance of both genes in roots slightly increased Upon salt treatment and after 3 days reached to their maximum levels and this expression continued until 8 days and then decreased to a basal expression similar to control for AlNHX gene but in AlSOS1 decreased to reach a new steady-state in which the mRNA content was about 2-fold that of control plants.
